期刊
CHEMOSPHERE
卷 73, 期 1, 页码 81-85出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2008.05.005
关键词
xenobiotics; 2,4-DCP; laccase; Box-Behnken design; biodegradation
资金
- Ministry of Human Resource development, Government of India
The effects of different environmental parameters, i.e., pH, temperature, time and enzyme concentration on the biodegradation of 2,4-dichlorophenol (2,4-DCP) in aqueous phase was evaluated with laccase from Pleurotus sp. using response surface methodology (RSM) in the present investigation. The Box-Behnken design of experiments was used to construct second order response surfaces with the investigated parameters. It was observed that the maximum degradation efficiency of similar to 98% was achieved at pH 6, temperature of 40 degrees C, time 9 h and an enzyme concentration of 8 IU ml(-1). The adequacy of the model was confirmed by the coefficient of multiple regression, R-2 and adjusted R-2 which were adjudged to be 87.9% and 73.6%, respectively indicating a reasonably good model for practical implementation. Despite the fact that many successful attempts have been taken in the past for biodegradation of 2,4-DCP using whole cells, the present study emphasizes the fastest biodegradation of 2,4-DCP, a potent xenobiotic compound. (c) 2008 Elsevier Ltd. All rights reserved.
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