期刊
CHEMMEDCHEM
卷 7, 期 1, 页码 79-84出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cmdc.201100457
关键词
aptamers; breast cancer; drug delivery; internalization; MCF-10AT1
资金
- National Key Scientific Program of China [2011CB911001, 2011CB911003]
- National Institutes of Health [GM066137, GM079359, CA133086]
Breast cancer affects one in eight women in the United States, with a mortality rate that is second only to lung cancer. Although chemotherapy is widely used in breast cancer treatment, its side effects remain a challenge. One way to address this problem is through drug delivery by the internalization of cell-type-specific probes. Although nucleic acid aptamers are excellent probes for molecular recognition, only a few studies have demonstrated that aptamers can be internalized into living cells. Therefore, herein we report the development of a cancer-cell-specific DNA aptamer probe, KMF2-1a. By using the cell-SELEX method, this aptamer was selected against breast cancer cell line MCF-10AT1. Our results show that KMF2-1a is internalized efficiently and specifically to the endosome of target breast cancer cells. These results indicate that KMF2-1a is a promising agent for cell-type-specific intracellular delivery with both diagnostic and therapeutic implications.
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