期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 9, 期 12, 页码 3586-3592出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201402808
关键词
bioimaging; dyes; pigments; fluorescent probes; FRET; hydrogen sulfide
资金
- MOST [2010CB126102]
- NSFC [21332004, 21402007]
- SRF for ROCS (SEM)
- Fundamental Research Funds for the Central Universities [YS1401]
- Public Hatching Platform for Recruited Talents of Beijing University of Chemical Technology
Hydrogen sulfide (H2S) is an endogenously produced gaseous signaling molecule with multiple biological functions. To visualize the endogenous in situ production of H2S in real time, new coumarin- and boron-dipyrromethene-based fluorescent turn-on probes were developed for fast sensing of H2S in aqueous buffer and in living cells. Introduction of a fluoro group in the ortho position of the aromatic azide can lead to a greater than twofold increase in the rate of reaction with H2S. On the basis of o-fluorinated aromatic azides, fluorescent probes with high sensitivity and selectivity toward H2S over other biologically relevant species were designed and synthesized. The probes can be used to in situ to visualize exogenous H2S and D-cysteine-dependent endogenously produced H2S in living cells, which makes them promising tools for potential applications in H2S biology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据