期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 9, 期 3, 页码 784-789出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201301326
关键词
aggregation-induced emission; carboxylesterase; fluorescence; self-assembly; tetraphenylethylene
资金
- National Science Fund for Distinguished Young Scholars [21125418]
- National Natural Science Foundation of China [21334004, 21344002]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Herein, we report a novel fluorescent light-up probe useful for carboxylesterase assay that is based on a tetraphenylethylene derivative containing carboxylic ester groups. The specific cleavage of the carboxylic ester bonds by carboxylesterase results in the generation of a relatively hydrophobic moiety that self-assembles into supramolecular microfibers, thus giving rise to turn-on fluorescent signals. A high sensitivity towards carboxylesterase was achieved with a detection limit as low as 29pM, which is much lower than the corresponding assays based on other fluorescent approaches.
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