Highly Selective and Sensitive Near-Infrared-Fluorescent Probes for the Detection of Cellular Hydrogen Sulfide and the Imaging of H2S in Mice

Herein, we report the development of two fluorescent probes for the highly selective and sensitive detection of H2S. The probes take advantage of a Cu(II)cyclen complex, which acts as a reaction center for H2S and as a quencher of BODIPY (boron-dipyrromethene)-based fluorophores with emissions at 765 and 680nm, respectively. These non-fluorescent probes could only be turned on by the addition of H2S, and not by other potentially interfering biomolecules, including reactive oxygen species, cysteine, and glutathione. In a chemical system, both probes detected H2S with a detection limit of 80nM. The probes were successfully used for the endogenous detection of H2S in HEK 293 cells, for measuring the H2S-release activity of dietary organosulfides in MCF-7 cells, and for the invivo imaging of H2S in mice.