期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 8, 期 1, 页码 276-281出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201200880
关键词
aggregation; alkaline phosphatase; biosensors; fluorescence; perylene probe
资金
- 100 Talents program of the Chinese Academy of Sciences
- National Natural Science Foundation of China [21075119, 91027036, 21275139]
- National Basic Research Program of China (973 Program) [2011CB911002]
- Pillar Program of Changchun Municipal Bureau of Science and Technology [2011225]
- Open Project of State Key Laboratory of Supramolecular Structure and Materials [SKLSSM201215]
A tetracationic perylene probe (probe 1) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.
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