期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 6, 期 12, 页码 3251-3259出版社
WILEY-BLACKWELL
DOI: 10.1002/asia.201100490
关键词
electron transfer; fluorescence; molecular beacons; oligonucleotides; peptide nucleic acids
资金
- Alexander von Humboldt Foundation
- Development and Promotion of Science and Technology Talents Project
- Thailand Research Fund
- Thai government [TKK2555, SP2]
- National Research University Grant from the Commission of Higher Education
- Ratchadaphiseksomphot Endowment [AM1006A]
A pyrene-labeled uridine (UPy) monomer for a pyrrolidinyl peptide nucleic acid with an alternating proline/2-aminocyclopentanecarboxylic acid backbone (acpcPNA) was synthesized and incorporated into the PNA. The UPy base in acpcPNA could specifically recognize the base A in its complementary DNA strand as determined by thermal denaturation (Tm) experiments. The fluorescence of the UPy-containing single-stranded acpcPNA was very weak in aqueous buffer. In the presence of a complementary DNA target, the fluorescence was enhanced significantly (2.741.9 folds, depending on sequences). The fluorescence enhancement was specific to the pairing between UPy and dA, making the UPy-modified acpcPNA useful as a hybridization-responsive fluorescence probe for DNA-sequence determination.
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