期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 6, 期 11, 页码 2956-2960出版社
WILEY-BLACKWELL
DOI: 10.1002/asia.201100712
关键词
DNA; enzymes; oligonucleotides; primers; reverse transcription
资金
- KAKENHI [20108015, 23550041]
- Takeda Science Foundation
- Banyu Life Science Foundation International
- Grants-in-Aid for Scientific Research [20108001, 20108015, 23550041, 10J05521] Funding Source: KAKEN
A phosphate-eliminated nonnatural oligonucleotide serves as a primer surrogate in reverse transcription reaction of mRNA. Despite of the nonnatural triazole linkages in the surrogate, the reverse transcriptase effectively elongated cDNA sequences on the 3'-downstream of the primer by transcription of the complementary sequence of mRNA. A structure-activity comparison with the reference natural oligonucleotides shows the superior priming activity of the surrogate containing triazole-linkages. The nonnatural linkages also protect the transcribed cDNA from digestion reactions with 5'-exonuclease and enable us to remove noise transcripts of unknown origins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据