期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 5, 期 1, 页码 123-129出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.200900468
关键词
bovine serum albumin; gold; microplates; nanostructures; proteins
资金
- NIH [DPI OD000798-04]
- NSF [DMR-0804088, ECS-0335765]
- Washington University in St. Louis
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP1OD000798] Funding Source: NIH RePORTER
- Division Of Materials Research [0804088] Funding Source: National Science Foundation
Gold microplates were synthesized in aqueous solutions by reducing HAuCl4 with the hydroxyl groups in both serine and threonine of bovine serum albumin (BSA), which is a globular protein in its native state. In this article, we systematically investigated the effects of temperature, pH value, the concentration of BSA, and ionic species on the reduction kinetics and thus the size and morphology of the final product. The optimal experimental conditions for producing uniform Au microplates include the following: an elevated temperature in the range of 55-65 degrees C, an acidic solution with pH approximate to 3, and the presence of NaCl (0.14 M). We found that if any one of these parameters was deviated from the optimal condition, Au microplates would not be formed in high yields. We also found that the surfaces of the as-synthesized Au microplates were covered by a dense array of BSA bumps.
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