期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 3, 期 8-9, 页码 1607-1612出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.200800099
关键词
biosensors; gold; peptides; photoaffinity labeling; surface plasmon resonance
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [17GS0221]
p38 mitogen-activated protein kinase (MAPK) is a member of the serine/threonine kinases and is activated in response to stress stimuli, such as cytokines, ultraviolet irradiation, heat shock, and osmotic shock. We revealed in a previous report that p62/SQSTM1, known to participate in proteasomal or autophagosomal protein degradation and cytokine receptor signal transduction pathways, binds to p38 to regulate specifically. Herein, we describe the improvement of the photoaffinity-thiol linker of our SPR imaging platform, which enabled us to determine the binding site of p62 to p38. SPR imaging experiments using a new photoaffinity linker 2 to immobilize the peptides derived from p62 on gold substrate indicate that the domain comprising amino acids 164-190 of p62 binds to p38 directly. These SPR analysis data and empirical biologic data reveal that the binding site of p62 to p38 is the domain corresponding to 173-182.
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