期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 24, 期 66, 页码 17434-17438出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201804151
关键词
biocatalysis; cascade reaction; enzymes; pantothenic acid
资金
- Netherlands Organization of Scientific Research (VICI) [724.016.002]
- European Research Council (PoC) [713483]
- Indonesia Endowment Fund for Education (LPDP)
- China Scholarship Council
- European Research Council (ERC) [713483] Funding Source: European Research Council (ERC)
Access to vitamin B-5 ((R)-pantothenic acid] and both diastereoisomers of alpha-methyl-substituted vitamin B-5 [(R)- and (S)-3-((R)-2,4-dihydroxy-3,3-dimethylbutanamido)-2-methylpropanoic acid) was achieved using a modular three-step biocatalytic cascade involving beta-methylaspartate ammonia lyase (MAL), aspartate-alpha-decarboxylase (ADC), beta-methylaspartate-alpha-decarboxylase (CrpG) or glutamate decarboxylase (GAD), and pantothenate synthetase (PS) enzymes. Starting from simple non-chiral dicarboxylic acids (either fumaric acid or mesaconic acid), vitamin B-5 and both diastereoisomers of alpha-methyl-substituted vitamin B-5, which are valuable precursors for promising antimicrobials against Plasmodium falciparum and multidrugresistant Staphylococcus aureus, can be generated in good yields (up to 70%) and excellent enantiopurity (> 99% ee). This newly developed cascade process may be tailored and used for the biocatalytic production of various vitamin B-5, derivatives by modifying the pantoyl or beta-alanine moiety.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据