期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 20, 期 24, 页码 7501-7506出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201400309
关键词
cascade reaction; detection; DNA recognition; enzyme mimicking; gold nanoparticle; sensors; V2O5 nanowire
资金
- 973 Project [2011CB936004, 2012CB720602]
- NSFC [21210002, 91213302]
Artificial enzyme mimics are a current research interest, and many nanomaterials have been found to display enzyme-mimicking activity. However, to the best of our knowledge, there have not hitherto been any reports on the use of pure nanomaterials to construct a system capable of mimicking an enzyme cascade reaction. Herein, we describe the construction of a novel nanocomposite consisting of V2O5 nanowires and gold nanoparticles (AuNPs) through a simple and facile chemical method, in which V2O5 and AuNPs possess intrinsic peroxidase and glucose oxidase (GOx)-like activity, respectively. Results suggest that this material can mimic the enzyme cascade reaction of horseradish peroxidase (HRP) and GOx. Based on this mechanism, a direct and selective colorimetric method for the detection of glucose has been successfully designed. Because single-strand and double-strand DNA (ssDNA and dsDNA) have different deactivating effects on the GOx-like activity of AuNPs, the sensing of target complementary DNA can also be realized and disease-associated single-nucleotide polymorphism of DNA can be easily distinguished. Our study opens a new avenue for the use of nanomaterials in enzyme mimetics, and holds promise for the further exploration of nanomaterials in creating alternative catalytic systems to natural enzymes.
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