期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 20, 期 49, 页码 16203-16209出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201404122
关键词
G-quadruplexes; nanostructures; machine; nucleic acid; switch; tweezers
资金
- Israel Science Foundation [1083/12]
Mimicking cellular transformations and signal transduction pathways by means of biocatalytic cascades proceeding in organized media is a scientific challenge. We describe two DNA machines that enable the ON/OFF switchable activation and deactivation of three-component biocatalytic cascades. One system consists of a reconfigurable DNA tweezers-type structure, whereas in the second system the catalytic cascade proceeds on a switchable DNA clamp scaffold. The three-component catalytic cascades consist of -galactosidase (-Gal), glucose oxidase (GOx), and the K+-ion-stabilized hemin-G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme. The hemin-G-quadruplex-bridged closed structure of the tweezers or clamp allows the biocatalytic cascades to operate (switched ON), whereas separation of the hemin-G-quadruplex by means of 18-crown-6-ether opens the tweezers/clamp structures, thus blocking the catalytic cascade (switched OFF). This study is complemented by two-component, switchable biocatalytic cascades composed of GOx and hemin-G-quadruplex assembled on hairpin-bridged DNA tweezers or clamp nanostructures.
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