Characterization of New Specific Copper Chelators as Potential Drugs for the Treatment of Alzheimer's Disease

The non-controlled redox-active metal ions, especially copper, in the brain of patients with Alzheimer disease (AD) should be considered at the origin of the intense oxidative damage in the AD brain. Several bis(8-aminoquinoline) ligands, such as 1 and PA1637, are able to chelate Cu2+ with high affinity, and are specific chelators of copper with respect to iron and zinc. They are able to efficiently extract Cu2+ from a metal-loaded amyloid. In addition, these tetradentate ligands are specific for the chelation of Cu2+ compared with Cu+. Consequently, the copper ion is easily released from the bis(8-aminoquinoline) ligand under reductive conditions, and can be trapped again by a protein having some affinity for copper such as human serum albumin (HSA) proteins. In addition, the copper is not efficiently released from [Cu(CQ)(2)] in reductive conditions. The catalytic production of H2O2 by [Cu2+-A(1-28)]/ascorbate is inhibited in vitro by the bis(8-aminoquinoline) 1, suggesting that 1 should be able to play a protective role against oxidative damages induced by copper-loaded amyloids.