Multivalent Recognition of Concanavalin A by {Mo132} Glyconanocapsules- Toward Biomimetic Hybrid Multilayers

Herein, we consider Muller's spherical, porous, anionic, molybdenum oxide based capsule, (NH4)(42)- [{(Mo-VI)(Mo5O21)-O-VI(H2O)(6)}(12){(Mo2O4)-O-V(CH3COO)}(30)]10CH(3)COONH(4) 300H(2)O(NH4)(42)1acrystal ingredients1, {Mo-132}, as an effective sugar-decorated nanoplatform for multivalent lectin recognition. The ion-exchange of NH4+ ions of 1 with cationic-sugars, D-mannose-ammonium chloride (2) or D-glucose-ammonium chloride (3) results in the formation of glyconanocapsules (NH4)(42-n)2(n)1a and (NH4)(42-m)3(m)1a. The Mannose (NH4)(42-n)2(n)1a capsules bind selectively ConcanavalinA (ConA) in aqueous solution, giving an association avidity constant of =21.9, reminiscent of the formation of glycoside clusters on the external surface of glyconanocapsule. The glyconanocapsules (NH4)(42-n)2(n)1a and (NH4)(42-m)3(m)1a self-assemble in hybrid multilayers by successive layer-by-layer deposition of (NH4)(42-n)2(n)1a or (NH4)(42-m)3(m)1a and ConA. These architectures, reminiscent of versatile mimics of artificial tissues, can be easily prepared and quantified by using quartz crystal microgravimetry (QCM). The biomimetic hybrid multilayers described here are stable under a continual water flow and they may serve as artificial networks for a greater depth of understanding of various biological mechanisms, which can directly benefit the fields of chemical separations, sensors or storage-delivery devices.