期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 19, 期 51, 页码 17552-17558出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201303029
关键词
click chemistry; dendrimers; gene technology; pillar[5]arenes; polycations
资金
- CNRS [UMR 7509, 7199]
- Agence Nationale de la Recherche (ANR)
- French Ministry of Research (MENRT)
Dendritic pillar[5]arene derivatives have been efficiently prepared by grafting dendrons with peripheral Boc-protected amine subunits onto a preconstructed pillar[5]arene scaffold. Upon cleavage of the Boc-protected groups, water-soluble pillar[5]arene derivatives with 20 (13) and 40 (14) peripheral ammonium groups have been obtained. The capability of these compounds to form stable nanoparticles with plasmid DNA has been demonstrated by gel electrophoresis, transmission electron microscopy (TEM), and dynamic light scattering (DLS) investigations. Transfection efficiencies of the self-assembled 13/pCMV-Luc and 14/pCMV-Luc polyplexes have been evaluated in vitro with HeLa cells. The transfection efficiencies found for both compounds are good, and pillar[5]arenes 13 and 14 show very low toxicity if any.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据