期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 19, 期 12, 页码 4036-4042出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201201677
关键词
bioorthogonal reactions; click chemistry; cyanobenzothiazole; DNA; post-synthesis modification
资金
- Molecular Basis of Disease Program at Georgia State University
- GSU University
- National Institutes of Health [GM086925, GM084933]
Post-synthesis modification of DNA is an important way of functionalizing DNA molecules. Herein, we describe a method that first enzymatically incorporates a cyanobenzothiazole (CBT)-modified thymidine. The side-chain handle CBT can undergo a rapid and site-specific cyclization reaction with 1,2-aminothiols to afford DNA functionalization in aqueous solution. Another key advantage of this method is the formation of a single stereo/regioisomer in the process, which allows for precise control of DNA modification to yield a single component for aptamer selection work and other applications.
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