期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 12, 页码 3444-3459出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201103364
关键词
enzyme assays; fluorescent dyes; macrocycles; molecular recognition; supramolecular chemistry
资金
- Deutsche Forschungsgemeinschaft (DFG) [NA-686/5, HE-5967/2-1]
- COST Action [CM1005]
- Fonds der Chemischen Industrie
We conceptualize a novel approach towards enzyme assays based on the reversible and competitive binding of a fluorescent dye and the substrate as well as product of an enzymatic reaction to a macrocyclic host. This method was termed supramolecular tandem assay, and has been applied to inhibitor and activator screening, sensor array development, and enantiomeric excess determination of amino acids. The simple and rapid read-out by fluorescence allows their straightforward implementation into high-throughput screening.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据