期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 45, 页码 14520-14526出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201201606
关键词
aminothiols; biological activity; fluorescent probes; FRET; native chemical ligation
资金
- NSFC [20872032, 20972044, 21172063]
- NCET [08-0175]
- Chinese Ministry of Education [20100161110008]
- Hunan University
Thiol-containing amino acids (aminothiols) such as cysteine (Cys) and homocysteine (Hcy) play a key role in various biological processes including maintaining the homeostasis of biological thiols. However, abnormal levels of aminothiols are associated with a variety of diseases. The native chemical ligation (NCL) reaction has attracted great attention in the fields of chemistry and biology. NCL of peptide segments involves cascade reactions between a peptide-a-thioester and an N-terminal cysteine peptide. In this work, we employed the NCL reaction mechanism to formulate a Forster resonance energy transfer (FRET) strategy for the design of ratiometric fluorescent probes that were selective toward aminothiols. On the basis of this new strategy, the ratiometric fluorescent probe 1 for aminothiols was judiciously designed. The new probe is highly selective toward aminothiols over other thiols and exhibits a very large variation (up to 160-fold) in its fluorescence ratio (I458/I603). The new fluorescent probe is capable of ratiometric detection of aminothiols in newborn calf and human serum samples and is also suitable for ratiometric fluorescent imaging of aminothiols in living cells.
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