期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 31, 页码 9552-9563出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201200597
关键词
chromophores; fluorescence; photochemistry; photophysics; sensors
资金
- Japan Science and Technology Agency (JST)
- Grants-in-Aid for Scientific Research [24107505, 23310157, 23500511] Funding Source: KAKEN
An environment-sensitive fluorophore can change its maximum emission wavelength (?em), fluorescence quantum yield (Ff), and fluorescence lifetime in response to the surrounding environment. We have developed two new intramolecular charge-transfer-type environment-sensitive fluorophores, DBThD-IA and DBSeD-IA, in which the oxygen atom of a well-established 2,1,3-benzoxadiazole environment-sensitive fluorophore, DBD-IA, has been replaced by a sulfur and selenium atom, respectively. DBThD-IA is highly fluorescent in n-hexane (Ff=0.81, ?em=537 nm) with excitation at 449 nm, but is almost nonfluorescent in water (Ff=0.037, ?em=616 nm), similarly to DBD-IA (Ff=0.91, ?em=520 nm in n-hexane; Ff=0.027, ?em=616 nm in water). A similar variation in fluorescence properties was also observed for DBSeD-IA (Ff=0.24, ?em=591 nm in n-hexane; Ff=0.0046, ?em=672 nm in water). An intensive study of the solvent effects on the fluorescence properties of these fluorophores revealed that both the polarity of the environment and hydrogen bonding with solvent molecules accelerate the nonradiative relaxation of the excited fluorophores. Time-resolved optoacoustic and phosphorescence measurements clarified that both intersystem crossing and internal conversion are involved in the nonradiative relaxation processes of DBThD-IA and DBSeD-IA. In addition, DBThD-IA exhibits a 10-fold higher photostability in aqueous solution than the original fluorophore DBD-IA, which allowed us to create a new robust molecular nanogel thermometer for intracellular thermometry.
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