期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 51, 页码 16556-16561出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201201468
关键词
fluorescence; nanohorns; nanotubes; peptides; protease
资金
- National Natural Science Foundation of China [21175126, 20875086]
Proteases play a central role in several widespread diseases. Thus, there is a great need for the fast and sensitive detection of various proteolytic enzymes. Herein, we have developed a carbon nanotube (CNT)-based protease biosensing platform that uses peptides as a fluorescence probe for the first time. Single-walled carbon nanohorns (SWCNHs) and thrombin were used to demonstrate this detection strategy. SWCNHs can adsorb a fluorescein-based dye (FAM)-labeled peptide (FAM-pep) and quench the fluorescence of FAM. In contrast, thrombin can cleave FAM-pep on SWCNHs and recover the fluorescence of FAM, which allows the sensitive detection of thrombin. This biosensor has a high sensitivity and selectivity toward thrombin, with a detection limit of 100 pM.
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