期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 14, 页码 4274-4284出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201103135
关键词
chloride sensing; crystal structures; fluorescence; macrocyclic ligands; NBD
资金
- Italian Ministero dell'Istruzione dell'Universita e della Ricerca (MIUR)
Ligand L (4-(7-nitrobenzo[1,2,5]oxadiazole-4-yl)-1,7-dimethyl-1,4,7,10-tetra-azacyclododecane) is a versatile fluorescent sensor useful for CuII, ZnII and CdII metal detection, as a building block of fluorescent metallo-receptor for halide detection, and as an organelle marker inside live cells. Ligand L undergoes a chelation-enhanced fluorescence (CHEF) effect upon metal coordination in acetonitrile solution. In all three complexes investigated the metal cation is coordinatively unsaturated; thus, it can bind secondary ligands as anionic species. The crystal structure of [ZnLCl](ClO4) is discussed. CuII and ZnII complexes are quenched upon halide interaction, whereas the [CdL]2+ species behaves as an OFFON sensor for halide anions in acetonitrile solution. The mechanism of the fluorescence response in the presence of the anion depends on the nature of the metal ion employed and has been studied by spectroscopic methods, such as NMR spectroscopy, UV/Vis and fluorescence techniques and by computational methods. Subcellular localization experiments performed on HeLa cells show that L mainly localizes in spot-like structures in a polarized portion of the cytosol that is occupied by the Golgi apparatus to give a green fluorescence signal.
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