期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 12, 页码 3589-3597出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201100634
关键词
calixarenes; circular dichroism; DNA recognition; fluorescent probes; hydrogen bonds; molecular recognition; UV; Vis spectroscopy
资金
- Deutsche Forschungsgemeinschaft
- Ministry of Science, Education and Sport of Croatia [098-0982914-2918, 098-0982464-2514]
A new class of potent DNA binding agents is presented. Dimeric calix[4]arenes with cationic groups at their upper rims and flexible alkyl bridges can be synthesized from triply acyl-protected calix[4]arene tetramines in relatively short synthetic sequences (35 steps). The compounds attach themselves to double-stranded nucleic acids in a noncovalent fashion, with micro- to nanomolar affinities. Guanidinium headgroups with their extended hydrogen-bonding fingers are more powerful than ammonium groups, and the benzylamine series is superior to the anilinium series (see below). The new ligands easily distinguish between RNA and various DNA types, and produce characteristic changes in UV/Vis, fluorescence, CD, as well as NMR spectra. Especially extended oligonucleotides of more than 100 base pairs are bound with affinities increasing from RNA (10 mu M Kd)
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