期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 25, 页码 7787-7792出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201200023
关键词
cell recognition; endocytosis; integrin; mesoporous materials; RGD
资金
- US National Science Foundation [CHE-0809521]
- US Department of Energy, Ames Laboratory, Office of Basic Energy Sciences [DE-AC02-07CH11358]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0809521] Funding Source: National Science Foundation
Recent breakthrough research on mesoporous silica nanoparticle (MSN) materials has illustrated their significant potential in biological applications due to their excellent drug delivery and endocytotic behavior. We set out to determine if MSN, covalently functionalized with conformation specific bioactive molecules (either linear or cyclic RGD ligands), behave towards mammalian cells in a similar manner as the free ligands. We discovered that RGD immobilized on the MSN surface did not influence the integrity of the porous matrix and improved the endocytosis efficiency of the MSN materials. Through competition experiments with free RGD ligands, we also discovered a conformation specific receptorintegrin association. The interaction between RGD immobilized on the MSN surface and integrins plays an important role in endosome trafficking, specifically dictating the kinetics of endosomal escape. Thus, covalent functionalization of biomolecules on MSN assists in the design of a system for controlling the interface with cancer cells.
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