期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 17, 期 38, 页码 10700-10707出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201101191
关键词
biosensors; magnetic properties; mesoporous materials; nanoparticles; peroxidase mimetics
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2009-0080602]
- NRF Converging Research Center [2009-0082276]
- KRIBB Research Initiative
- Industry, Academy, and Research Institute
- Korea Small and Medium Business Administration [00043279]
- POSTECH Basic Science Research Institute
- National Research Foundation of Korea [2009-0080602] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A nanostructured multicatalyst system consisting of Fe3O4 magnetic nanoparticles (MNPs) as peroxidase mimetics and an oxidative enzyme entrapped in large-pore-sized mesoporous silica has been developed for convenient colorimetric detection of biologically important target molecules. The construction of the nanocomposites begins with the incorporation of MNPs on the walls of mesocellular silica pores by impregnating Fe(NO3)(3)center dot 9H(2)O, followed by the immobilization of oxidative enzymes. Glutaraldehyde cross-linking was employed to prevent enzymes leaching from the pores and led to over 20 wt% loading of the enzyme. The oxidase in the nanocomposite generates H2O2 through its catalytic action for target molecules and subsequently activates MNPs to convert selected substrates into colored products. Using this strategy, two different biosensing systems were constructed employing glucose oxidase and cholesterol oxidase and their analytical capabilities were successfully verified by colorimetrically detecting the corresponding target molecules with excellent selectivity, sensitivity, reusability, and stability. Future potential applications of this technology range from biosensors to multicatalyst reactors.
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