期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 17, 期 34, 页码 9377-9384出版社
WILEY-BLACKWELL
DOI: 10.1002/chem.201100478
关键词
biosensors; enzyme catalysis; helical carbon nanotubes; nanoparticles; peroxidase nanomimetics
资金
- National Natural Science Foundation of China [20905010, 51001019, 20821063]
- National Basic Research Program of China [2011CB933502]
- China Postdoctoral Science Foundation [20100471293]
A combined hydrothermal/hydrogen reduction method has been developed for the mass production of helical carbon nanotubes (HCNTs) by the pyrolysis of acetylene at 475 degrees C in the presence of Fe(3)O(4) nanoparticles. The synthesized HCNTs have been characterized by high-resolution transmission electron microscopy, scanning electron microscopy, X-ray diffraction analysis, vibrating sample magnetometry, and contact-angle measurements. The as-prepared helical-structured carbon nanotubes have a large specific surface area and high peroxidase-like activity. Catalysis was found to follow Michaelis-Menten kinetics and the HCNTs showed strong affinity for both H(2)O(2) and 3,3', 5,5',-tetramethylbenzidine (TMB). Based on the high activity, the HCNTs were firstly used to develop a biocatalyst and amperometric sensor. At pH 7.0, the constructed amperometric sensor showed a linear range for the detection of H(2)O(2) from 0.5 to 115 mu m with a correlation coefficient of 0.999 without the need for an electron-transfer mediator. Because of their low cost and high stability, these novel metallic HCNTs represent a promising candidate as mimetic enzymes and may find a wide range of new applications, such as in biocatalysis, immunoassay, and environmental monitoring.
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