期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 17, 期 12, 页码 3465-3473出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201002031
关键词
ion channels; membranes; molecular recognition; self assembly; vesicles
资金
- EPSRC [EP/C004396/1]
- UKRC for Women in Science, Engineering and Technology
- Engineering and Physical Sciences Research Council [EP/C004396/1] Funding Source: researchfish
Simple synthetic methodology has been used to create biotinylated pyridyl cholate lipids that can undergo multiple self-assembly events when inserted into phospholipid vesicles; Pd-II links cholates into transmembrane lipids, while avidin laterally clusters these complexes together and concomitantly assembles the vesicles into aggregates. The transmembrane assembly of cholates by Pd-II opened the ion channels, whereas avidin addition produced vesicle aggregates, giving a system that mimicked both transmembrane transport and cellular adhesion. Complexation of these Pd-II-linked cholates by avidin gave a measurable decrease in ion flow, suggesting some channels became blocked or were prevented from adopting the optimum geometry for ion conduction. This reflects the importance of spatially appropriate preorganisation when generating active supramolecular assemblies.
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