期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 17, 期 31, 页码 8657-8662出版社
WILEY-BLACKWELL
DOI: 10.1002/chem.201003372
关键词
DNA; enzymes; nanowires; polymorphism; Raman spectroscopy
资金
- Ministry of Knowledge Economy through IITA
- MEST
- Avellino Co.
- NRF of Korea [2010-0018868]
- MEST, Korea [2011K000210]
- National Research Foundation of Korea [과C6A1907] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Single nucleotide polymorphisms (SNPs) can serve as important biomarkers for genetic diseases, for which accurate detection of SNPs is essential for early diagnosis. We have developed a novel SNP sensor by combining a Au nanowire-on-film surface-enhanced Raman scattering (SERS) platform with S1 nuclease reaction. The combined sensor system provides reproducible SERS signals only in the presence of perfectly matched target DNAs, to probe DNAs as a result of single-stranded DNA-specific degradation by S1 nuclease. Furthermore, point mutations in DNA causing Wilson disease and Avellino corneal dystrophy were successfully identified by this sensor, thereby indicating its practical ability to diagnose genetic diseases.
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