期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 16, 期 2, 页码 568-572出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200901591
关键词
fluorescence; fluorescence spectroscopy; sensors; transition metals; zinc
资金
- Ministry of Education. Culture. Sports, Science and Technology of Japan [20689001, 19890047, 21659024]
- Mochida Menimial Foundation
- Medical and Pharmaceutical Research and Sankyo Foundation of Life Science
Fluorescence imaging is a powerful tool for the visualization of biological molecules in living cells, tissue slices, and whole bodies, and is important for elucidating biological phenomena. Furthermore, zinc (Zn2+) is the second most abundant heavy metal ion ill the human body after iron, and detection of chelatable Zn2+ in biological studies has attracted much attention. Herein, we present a novel, highly sensitive off on fluorescent chemosensor for Zn2+ by using the internal charge transfer (ICT) mechanism. The rationale of our approach to highly sensitive sensor molecules is as follows. If fluorescence can be completely quenched in the absence of Zn2+, chemosensors would offer a better signal-to-noise ratio. However, it is difficult to quench the fluorescence completely before Zn2+ binding, and most sensor molecules still show very weak fluorescence in the absence of Zn2+. But even though the sensor shows a weak fluorescence in the absence of Zn2+, this fluorescence call be further suppressed by selecting an excitation wavelength that is barely absorbed by the Zn2+-free sensor molecule. Focusing oil careful control of ICT within the 4-amino-1,8-naphthalimide dye platform, we designed and synthesized a new chemosensor (1) that shows a pronounced fluorescence enhancement with a blueshift in the absorption spectrum upon addition of Zn2+. The usefulness of I for monitoring Zn2+ changes was confirmed in living HeLa cells. There have been several reports on 4-amino-1,8-naphthalimide-based fluorescent sensor molecules. However, I is the first Zn2+-sensitive off-on fluorescent sensor molecule that employs the ICT mechanism; most off-on sensor molecules for Zn2+ employ the photoinduced electron transfer (PeT) mechanism.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据