期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 16, 期 1, 页码 104-113出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200902202
关键词
fluorescence spectroscopy; indicator displacement assay; organometallic complexes; peptides; sensors
资金
- Swiss National Science Foundation
- China Scholarship Council (CSC)
- Claude & Giuliana Foundation
- EPFL
A simple but powerful method for the sensing of peptides in aqueous solution has been developed. The transition-metal complexes [PdCl2(en)], [{RhCl2Cp*}(2)], and [{RuCl2(p-cymene)}(2)] were combined with six different fluorescent dyes to build a cross-reactive sensor array. The fluorescence response of the individual sensor units was based on competitive complexation reactions between the peptide analytes and the fluorescent dyes. The collective response of the sensor array in a time-resolved fashion was used as an input for multivariate analyses. A sensor array comprised of only six metal-dye combinations was able to differentiate ten different dipeptides in buffered aqueous solution at a concentration of 50 mu M. Furthermore, the cross-reactive sensor could be used to obtain information about the identity and the quantity of the pharmacologically interesting dipeptides carnosine and homocarnosine in a complex biological matrix, such as deproteinized human blood serum. The sensor array was also able to sense longer peptides, which was demonstrated by differentiating mixtures of the nonapeptide bradykinin and the decapeptide kallidin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据