期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 15, 期 44, 页码 11898-11903出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200901275
关键词
aptamers; DNA machine; DNA replication; inhibitors; proteins
资金
- National Natural Science Foundation of China [20873175, 20805055, 20725516]
- Ministry of Science and Technology [2006CB933000, 2007CB936000, 2007AA06A406]
- Ministry of Health [2009ZX10004-301]
- Shanghai Municipal Commission for Science and Technology [0752nm021, 0952nm04600, 0939191500]
DNA replication plays a central role in living organisms. Unregulated or uncontrollable DNA replication is well known to result in many pathological states, such as cancer, autoimmune diseases, and viral/bacterial infections. We report that an aptamer-protein complex could indirectly inhibit in vitro replication of DNA. An isothermal DNA machine based on the strand-displacement amplification is employed to support our assumption. An antithrombin aptamer sequence is rationally encoded into the DNA replication template. Once thrombin binds to the template, the as-formed aptamer-protein complexes can, in turn, become a barrier to the polymerase and inhibit the DNA replication activities in both static and dynamic modes. The inhibition is successfully confirmed by both fluorescence and gel-electrophoresis experiments. Considering the availability of a broad library of aptamers and the existence of various DNA/protein interactions, our results imply the possibility for the rational regulation of DNA replication in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据