期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 15, 期 18, 页码 4704-4709出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200801722
关键词
gold; immunoassays; luminescence; magnetic beads; nanoparticles
资金
- National Natural Science Foundation of China20775038 [20775038]
- National High-tech RD Program [2007AA09Z113]
- Excellent Young Scientists Foundation of Shandong Province [JQ200805]
A novel and sensitive chemiluminescence immunoassay (CLIA) has been developed by employing a new chemiluminescence (CL) enhancer, bromophenol blue (BPB), for the determination of alpha-fetoprotein (AFP) based on magnetic beads (MBs) and colloidal gold nanoparticles (AuNPs) modified with HRP-labeled anti-AFP antibodies. BPB. as a chemical indicator, was found to act as a novel and highly signal enhancer of the peroxidase-catalyzed CL reaction of lurninol with hydrogen peroxide. After optimizing the CL reaction conditions, this new luminol-H2O2-HRP-BPB CL system was applied to a sandwich-type CLIA based on the magnetic separation and the amplification feature of AuNPs as HRP labels. A linear range was obtained when the concentrations of AFP were from 0.1. to 5.0 ng mL(-1) (R=0.9997) with the detection limit of 0.01 ngmL(-1) (3 sigma), which is one order of magnitude lower than that obtained without using AuNPs, and much lower than that typically achieved by ELISA. The present method was successfully applied to the determination of AFP in human serum samples. The results indicated that this proposed protocol could be quite promising for the application in immunoassays.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据