期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 14, 期 29, 页码 8922-8927出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200800432
关键词
chelates; cooperativity; emission spectroscopy; molecular recognition; peptides
资金
- Volkswagen Foundation
- Fonds der Chemischen Industrie
- University of Regensburg
- Graduiertenkolleg GRK 760
Artificial ditopic receptors for the differentiation of phosphorylated peptides varying in i+3 amino acid side chains were synthesized, and their binding affinities and selectivities were determined. The synthetic receptors show the highest binding affinities to phosphorylated peptides under physiological conditions (HEPES, pH 7.5, 154 mm NaCl) reported thus far for artificial systems. The tight and selective binding was achieved by high cooperativity of the two binding moieties in the receptor molecules. All receptors interact with phosphorylated serine by bis(Zn-II-cyclen) complex coordination and a second binding site recognizing a carboxylate or imidazole amino acid side chain functionality.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据