期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 5, 页码 1723-1727出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201509649
关键词
fluorophore; microscopy; photoactivation; Si-rhodamine; super-resolution imaging
资金
- Howard Hughes Medical Institute
- German Ministry of Research and Education [13N12781]
The rhodamine system is a flexible framework for building small-molecule fluorescent probes. Changing N-substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si-containing analogue of Q-rhodamine. This probe is the first example of a caged Si-rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red-shifted to allow multicolor imaging. The dye is a useful label for super-resolution imaging and constitutes a new scaffold for far-red fluorogenic molecules.
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