期刊
CHEMISTRY AND PHYSICS OF LIPIDS
卷 165, 期 5, 页码 545-562出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2012.06.001
关键词
Lipidomics; MALDI imaging; Traumatic brain injury; Acute lung injury; Lipid peroxidation; Docosahexaenoic acid
资金
- NIH [U19AI068021, HL70755, HL094488, ES020693, ES021068, NS076511]
- Cancer Center Support Grant (CCSG) [P30 CA047904]
- NIOSH [OH008282]
- US Army [W81XWH-09-2-0187]
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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