期刊
CHEMISTRY & BIOLOGY
卷 19, 期 8, 页码 1041-1048出版社
CELL PRESS
DOI: 10.1016/j.chembiol.2012.07.007
关键词
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资金
- Canadian Institutes of Health Research [MOP-64300, MOP-84239]
- Hans Se lye Research Chair (Bristol-Myers Squibb)
- Groupe d'Etudes des Proteines Membranaires
- Fonds de Recherche du Quebec-Sante
- Canada Foundation for Innovation
Secretion systems translocate virulence factors of many bacterial pathogens, enabling their survival inside the host organism. Consequently, inhibition strongly attenuates pathogenicity and can be considered a target for novel antimicrobial drugs. The type IV secretion system (T4SS) of the intracellular pathogen Brucella is a prerequisite for its virulence, and in this work we targeted the interactions of the essential assembly factor protein, VirB8, using small-molecule inhibitors. High-throughput screening identified several potent and specific inhibitors, and the target-binding site of these inhibitors was identified by X-ray crystallography, in silico docking, and analysis of the derivates of the inhibitor B8I-2. VirB8 interaction inhibitors bind to a surface groove opposite to the dimerization interface, and by varying the binding-site residues, we were able to determine which residues are required for inhibitor activity. E115 and K182 were found to be especially important, and changes at R114, Y229, and L151 also reduced inhibitor efficiency.
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