期刊
CHEMISTRY & BIOLOGY
卷 18, 期 3, 页码 372-380出版社
CELL PRESS
DOI: 10.1016/j.chembiol.2010.12.019
关键词
-
资金
- Air Force Office of Scientific Research
- MURI [FA9550-08-1-0145]
- DOE Bioenergy Science Center (BESC)
- USDA Biodesign and Bioprocess Center
- ICTAS
Different from NAD(P)H regeneration approaches mediated by a single enzyme or a whole-cell microorganism, we demonstrate high-yield generation of NAD(P)H from a renewable biomass sugar-cellobiose through in vitro synthetic enzymatic pathways consisting of 12 purified enzymes and coenzymes. When the NAD(P)H generation system was coupled with its consumption reaction mediated by xylose reductase, the NADPH yield was as high as 11.4 mol NADPH per cellobiose (i.e., 95% of theoretical yield-12 NADPH per glucose unit) in a batch reaction. Consolidation of endothermic reactions and exothermic reactions in one pot results in a very high energy-retaining efficiency of 99.6% from xylose and cellobiose to xylitol. The combination of this high-yield and projected low-cost biohydrogenation and aqueous phase reforming may be important for the production of sulfur-free liquid jet fuel in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据