期刊
CHEMISTRY & BIOLOGY
卷 16, 期 11, 页码 1140-1147出版社
CELL PRESS
DOI: 10.1016/j.chembiol.2009.10.010
关键词
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资金
- RIKEN
- Molecular Ensemble Development Research Team
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- New Energy and Industrial Technology Development Organization
- Human Frontier Science
KikGR is a fluorescent protein engineered to display green-to-red photoconvertibility that is induced by irradiation with ultraviolet or violet light. Similar to Kaede and EosFP, two naturally occurring photoconvertible proteins, KikGR contains a HiS(62)-Tyr(63)-Gly(64) tripeptide sequence, which forms a green chromophore that can be photoconverted to a red one via formal beta-elimination and subsequent extension of a pi-conjugated system. Using a crystallizable variant of KikGR, we determined the structures of both the green and red state at 1.55 angstrom. resolution. The double bond between HiS(62)-C-alpha and HiS(62)-C-beta in the red chromophore is in a cis configuration, indicating that rotation along the HiS(62) C-alpha-C-beta bond occurs following cleavage of the HiS(62) N-alpha-C-alpha bond. This structural rearrangement provides evidence that the beta-elimination reaction governing the green-to-red photoconversion of KikGR follows an E1 (elimination, unimolecular) mechanism.
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