期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 203, 期 2, 页码 377-385出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2013.01.004
关键词
Spermatozoa motility; DNA damage; Oxidative stress; ATP; Sturgeon
资金
- [IAA 608030801]
- [ME10015]
- [QH82119]
- [CZ.1.05/2.1.00/01.0024]
- [GACR P502/11/0090]
- [GAJU 046/2010/Z]
The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 mu/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 mu g/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 mu/l and above. After 2 h exposure at higher test concentrations (10-50 mu/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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