期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 171, 期 1, 页码 89-95出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2007.09.001
关键词
epigallocatechin-3-gallate; extracellular signal-regulated kinase; prostate cancer; green tea
Epigallocatechin-3-gallate (EGCG), a tea polyphenol, inhibits the proliferation of many cancer cell lines; however, the antiproliferative mechanism(s) are not well-characterized. The objective of this study is to identify the cellular signaling mechanism(s) responsible for the anti proliferative effects of EGCG in the PC-3 prostate cancer cell line. EGCG inhibited PC-3 cell proliferation in a concentration-dependent manner with an IC50 value of 39.0 mu M, but had no effect on the proliferation of a nontumorigenic prostate epithelial cell line (RWPE-1). Treatment of PC-3 cells with EGCG (0-50 mu M) resulted in time and concentration-dependent activation of the extracellular signal-regulated kinase (ERK1/2) pathway. EGCG treatment did not induce ERK1/2 activity in RWPE-1 cells. The activation of ERK1/2 by EGCG was not inhibited using PD98059, a potent inhibitor of mitogen-activated protein kinase kinase (MEK), the immediate upstream kinase responsible for ERK1/2 activation; suggesting a MEK-independent signaling mechanism. Pretreatment of PC-3 cells with a phosphomositide-3 kinase (PI3K) inhibitor partially reduced both EGCG-induced ERK1/2 activation and the anti proliferative effects of this polyphenol. These results suggest that ERK1/2 activation via a MEK-independent, PI3-K-dependent signaling pathway is partially responsible for the anti proliferative effects of EGCG in PC-3 cells. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
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