期刊
CHEMICAL SOCIETY REVIEWS
卷 43, 期 4, 页码 1189-1200出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cs60208g
关键词
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资金
- University of Wisconsin-Madison
- Wisconsin Alumni Research Foundation (WARF)
- Department of Biochemistry
- National Institutes of Health [K99/R00, R00 GM086471]
- Arnold and Mabel Beckman Foundation
- Hatch Act Formula Fund from the USDA [WIS 01625]
- Molecular Biophysics Training Program [NIH T32-GM08293]
Many of the cell's macromolecular machines contain multiple components that transiently associate with one another. This compositional and dynamic complexity presents a challenge for understanding how these machines are constructed and function. Colocalization single molecule spectroscopy enables simultaneous observation of individual components of these machines in real-time and grants a unique window into processes that are typically obscured in ensemble assays. Colocalization experiments can yield valuable information about assembly pathways, compositional heterogeneity, and kinetics that together contribute to the development of richly detailed reaction mechanisms. This review focuses on recent advances in colocalization single molecule spectroscopy and how this technique has been applied to enhance our understanding of transcription, RNA splicing, and translation.
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