期刊
CHEMICAL SOCIETY REVIEWS
卷 38, 期 10, 页码 2833-2841出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b907749a
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资金
- National Institute of Health [NIDDK 1RO1DK079109-01]
- Department of Energy [DE-FG02-04ER15542]
- Canada Research Chairs program, NSERC
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK079109] Funding Source: NIH RePORTER
Fluorescent proteins have revolutionized cell biology by allowing researchers to non-invasively peer into the inner workings of cells and organisms. While the most common applications of fluorescent proteins are to image expression, localization, and dynamics of protein chimeras, there is a growing interest in using fluorescent proteins to create biosensors for minimally invasive imaging of concentrations of ions and small molecules, the activity of enzymes, and changes in the conformation of proteins in living cells. This tutorial review provides an overview of the progress made in the development of fluorescent protein-based biosensors to date.
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