期刊
CHEMICAL SENSES
卷 35, 期 8, 页码 663-673出版社
OXFORD UNIV PRESS
DOI: 10.1093/chemse/bjq055
关键词
dgq; plc21C; proboscis; rdgA; taste transduction; wing margins
资金
- National Centre for Biological Sciences
- Department of Biotechnology
In Drosophila melanogaster, gustatory receptor genes (Grs) encode putative G-protein-coupled receptors (GPCRs) that are expressed in gustatory receptor neurons (GRNs). One of the Gr genes, Gr5a, encodes a receptor for trehalose that is expressed in a subset of GRNs. Although a role for the G protein, Gs alpha, has been shown in Gr5a-expressing taste neurons, there is the residual responses to trehalose in Gs alpha mutants which could suggest additional transduction mechanisms. Expression and genetic analysis of the heterotrimeric G-protein subunit, Gq, shown here suggest involvement of this G alpha subunit in trehalose perception in Drosophila. A green fluorescent protein reporter of Gq expression is detected in gustatory neurons in the labellum, tarsal segments, and wing margins. Animals heterozygous for dgq mutations and RNA interference-mediated knockdown of dgq showed reduced responses to trehalose in the proboscis extension reflex assay and feeding behavior assay. These defects were rescued by targeted expression of the wild-type dgq alpha transgene in the GRNs. These data together with observations from other mutants in phospholipid signaling provide insights into the mechanisms of taste transduction in Drosophila.
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