期刊
CHEMICAL SENSES
卷 34, 期 6, 页码 503-512出版社
OXFORD UNIV PRESS
DOI: 10.1093/chemse/bjp027
关键词
antennal cDNA library; expression pattern; Microplitis mediator; odorant-binding proteins
资金
- National Natural Science Foundation of China [30871640, 30330410]
- China national 973 Basic Research Program [2007CB109202]
- Research Foundation of State Key Laboratory for Biology of Plant Diseases and Insect Pests [SKL2007SR01]
The parasitoids of Cotton Bollworm Microplitis mediator (Hymenoptera: Braconidae) find their hosts through the odor released by stressed plants. In this study, preliminary characterization and isolation of cDNAs from male M. mediator antennal libraries identified 8 putative odorant-binding proteins (OBPs). Real-time polymerase chain reaction method was used to study the expression pattern of these isolated genes. Their gene expression profiles under a wide range of conditions indicated that only 4 OBP genes in M. mediator were antenna specific. The remaining 4 genes are either expressed ubiquitously or strictly regulated in specialized tissues or during different developmental stages. Some OBP genes were gender specific. These findings support that OBPs play dynamic roles during development of M. mediator and are likely to be involved in broader physiological functions.
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