期刊
CHEMICAL RESEARCH IN TOXICOLOGY
卷 22, 期 11, 页码 1841-1850出版社
AMER CHEMICAL SOC
DOI: 10.1021/tx9002512
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资金
- Consejeria de Educacion y Ciencia de la Junta de Comunidades de Castilla-La Mancha (JCCM, Spain) [PAI-05-036, PAI08-0175-8618]
- JCCM
Oxidation of acetaminophen by human methemoglobin in the presence of H2O2 has been kinetically studied in the present paper. The drug showed a protective effect against the H2O2-induced irreversible inactivation of the protein, thus indicating the competition among both ligands, H2O2 and acetaminophen for the protein. The stoichiometry of the reaction is variable and depends on relative initial concentrations of H2O2 and the drug owing to their competitive behavior. In addition and unexpectedly, the protein exhibits non Michaelian kinetics against both acetaminophen and H2O2-under steady-state conditions and shows negative co-operativity with Hill coefficients in the 0.3-0.7 range. Therefore, these data were compared to those obtained with myoglobin under similar experimental conditions, and the same results were observed. This led its to propose a mechanism for the peroxidase-like activity of hemoglobin, which accounts for the experimental results obtained herein. The steady-state rate equation for this mechanism has been obtained and is also consistent with the experimental data, thus indicating the goodness of the model proposed herein. The results presented in this work provide new insights into the oxidation mechanism of acetaminophen.
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