期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 292, 期 -, 页码 98-107出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2015.03.023
关键词
Carbon nanotubes; Diameter; Serum albumin; Nanotoxicology
资金
- Cultivation Fund of the Key Scientific and Technical Innovation Project
- Research Fund for the Doctoral Program of Higher Education
- Ministry of Education of China [708058, 20130131110016]
- Science and Technology Development Plan of Shandong Province, China [2014GSF117027]
- [20875055]
- [21277081]
- [21477067]
In this work, we investigated and compared carbon nanotubes (CNTs) of different diameters regarding their interaction with bovine serum albumin (BSA) and their ability to alter protein structure. BSA was exposed to CNT solutions, and the effects were assessed by utilizing fluorescence spectroscopy, UV-vis absorption spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), bichinchoninic acid (BCA) and zeta-potential measurement assays. We demonstrate that CNT diameter and surface area play key roles in influencing the stability of adsorbed proteins. Results showed that the secondary and tertiary structural stability of BSA decreased upon adsorption onto CNTs, with greater decrease on smaller-diametered nanotubes. Besides, more protein was loaded onto CNTs with small diameter, reducing the cytotoxicity. This study, therefore, provides fundamental information for the influence of CNT diameter and surface on protein behavior, which may be helpful to understand toxic effects of CNTs and prove beneficial for developing novel biomedical devices and safe use of nanomaterials. (C) 2015 Elsevier B.V. All rights reserved.
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