期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 285, 期 -, 页码 11-17出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2014.11.018
关键词
Aflatoxin B1; Mycotoxin; Surface-enhanced Raman scattering; Silica-encapsulation; Magnetic bead
资金
- National Research Foundation of Korea [K20904000004-13A0500-00410]
- BioNano Health-Guard Research Center - Ministry of Science, ICT and Future Planning of Korea, Global Frontier Project [2013M3A6B2078957]
- National Research Foundation of Korea [2013M3A6B2078957] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Aflatoxin B1 (AFB1) is a well-known carcinogenic contaminant in foods. It is classified as an extremely hazardous compound because of its potential toxicity to the human nervous system. AFB1 has also been extensively used as a biochemical marker to evaluate the degree of food spoilage. In this study, a novel surface-enhanced Raman scattering (SERS)-based immunoassay platform using silica-encapsulated hollow gold nanoparticles (SEHGNs) and magnetic beads was developed for highly sensitive detection of AFB1.SEHGNs were used as highly stable SERS-encoding nano tags, and magnetic beads were used as supporting substrates for the high-density loading of immunocomplexes. Quantitative analysis of AFB1 was performed by monitoring the intensity change of the characteristic peaks of Raman reporter molecules. The limit of detection (LOD) of AFB1, determined by this SERS-based immunoassay, was determined to be 0.1 ng/mL. This method has some advantages over other analytical methods with respect to rapid analysis (less than 30 min), good selectivity, and reproducibility. The proposed method is expected to be a new analytical tool for the trace analysis of various mycotoxins. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据