In vivo ratiometric Zn2+ imaging in zebrafish larvae using a new visible light excitable fluorescent sensor

A visible light excitable ratiometric Zn2+ sensor was developed by integrating a Zn2+ chelator as the ICT donor of the fluorophore sulfamoylbenzoxadiazole, which displays the Zn2+-induced hypsochromic emission shift (40 nm) and favors the in vivo ratiometric Zn2+ imaging in zebrafish larvae.