eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions

We report a simple electrochemical method referred to as eMethylsorb for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.