期刊
CHEMICAL COMMUNICATIONS
卷 50, 期 86, 页码 13153-13156出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc06732k
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资金
- National Breast Cancer Foundation of Australia (NBCF) National Collaborative Research Grant [CG-12-07]
- ARC DECRA [DE120102503]
- DP [DP140104006]
- UQ Fellowship [2012001456]
- Australian Research Council [DE120102503] Funding Source: Australian Research Council
We report a simple electrochemical method referred to as eMethylsorb for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.
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