A diazirine-based photoaffinity probe for facile and efficient aptamer-protein covalent conjugation

A photo-reactive functional labelling reagent, diazirine phosphoramidite, was designed and synthesized for easy and flexible sitespecific labelling of oligonucleotides with the diazirine moiety. The new reagent allows facile photo-crosslinking of oligonucleotide with its interacting partner for a variety of applications, including tertiary structure determination, molecular interaction study and biomarker discovery.